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Search Purification (Commentary)

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Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
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EC Number Purification (Commentary)
Show all pathways known for 1.14.13.83Display the reaction diagram Show all sequences 1.14.13.83-
Show all pathways known for 1.14.13.83Display the reaction diagram Show all sequences 1.14.13.83centrifugation of cells, pellet resuspended in binding buffer (20 mM Tris-HCl, pH 8.0, containing 0.5 M NaCl and 5 mM imidazole), cells broken up with a cell disrupter or sonicated, centrifugation, supernatant transferred to an anaerobic glove box, applied to a metal affinity chromatography column charged with Ni2+, washed with buffer with 20 mM imidazole, elution with 400 mM imidazole
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