EC Number   |
|---|
    1.14.13.122 | - |
| 1.14.13.122 | for immunoblotting analysis rosette leaves are homogenized with extraction buffer (50 mM Tris, pH 6.8, 2 mM EDTA, 10% glycerol, 2% SDS, 6% 2-mercaptoethanol), centrifugation, supernatant separated by SDS-PAGE, resolved proteins electroblotted onto Hybond-P membrane, identification of fusion enzyme signal with antibody-horseradish peroxidase system |
| 1.14.13.122 | partially by purification of chloroplast envelope inner membrane and thylakoid membranes |
| 1.14.13.122 | recombinant enzyme partially by purification of transgenic plant thylakoid membranes |
| 1.14.13.122 | refolded recombinant His6-tagged CAO from Escherichia coli by nickel affinity chromatography to about 85% purity |
| 1.14.13.122 | rosette leaves are homogenized with extraction buffer (50 mM Tris, pH 6.8, 2 mM EDTA, 10% glycerol, 2% SDS, 6% 2-mercaptoethanol), centrifugation, supernatants separated by SDS-PAGE, proteins electroblotted onto a Hybond-P membrane, identification of fusion enzyme with antibody-horseradish peroxidase system |
