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EC Number
Purification (Commentary)
Reference
1.14.13.1
-
389998
,
389999
,
390000
,
390005
,
390009
,
439423
1.14.13.1
affinity chromatography
390008
1.14.13.1
cells grown on 1-hydroxy-2-naphthoate
390017
1.14.13.1
DEAE-Toyopearl 650M column chromatography, phenyl Sepharose CL6B column chromatography, and Sephacryl gel filtration
741666
1.14.13.1
HisTrap column chromatography
742130
1.14.13.1
native enzyme from 5-chlorosalicylate-grown cells by anion exchange chromatography and gel filtration
680483
1.14.13.1
Ni-NTA column chromatography and Superdex 75 gel filtration
741825
1.14.13.1
Ni-NTA His Bind resin column chromatography
675817
1.14.13.1
oxygenase component PhnII
684569
1.14.13.1
recombinant expression of His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography, tag cleavage and a second step of nickel affinity chromatography
764872
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