EC Number   |
|---|
   1.14.11.15 | - |
| 1.14.11.15 | C18-reverse HPLC column chromatography |
| 1.14.11.15 | high performance liquid chromatography |
| 1.14.11.15 | immobilised metal affinity chromatography and Superdex 200 gel filtration |
| 1.14.11.15 | partial |
| 1.14.11.15 | recombinant maltose-binding protein-fused enzyme from Escherichia coli by amylose affinity chromatography |
| 1.14.11.15 | rosette leaves are frozen in liquid nitrogen and ground to a fine powder, protein extracted with 50 mM Tris-HCl, pH 8.0, containing 200 mM NaCl, 5 mM EDTA, 0.1% Tween 20 and a proteinase inhibitor mixture, SDS-PAGE |
| 1.14.11.15 | transgenic plants are ground in liquid nitrogen, total cellular extracts suspended in 1X SDS-PAGE sample loading buffer |
