EC Number |
---|
1.14.11.1 | - |
1.14.11.1 | partial |
1.14.11.1 | recombinant His-tagged enzyme by nickel affinity chromatography, gel filtration, ultrafiltration, and buffer exchange via desalting gel filtration |
1.14.11.1 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and anion exchange chromatography |
1.14.11.1 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and anion exchange chromatography, to over 90% purity |
1.14.11.1 | recombinant His-tagged GBBH from Escherichia coli strain WK6 by nickel affinity chromatography |
1.14.11.1 | recombinant His6-tagged enzyme from Saccharomyces cerevisiae strain AH22 by nickel affinity chromatography, desalting gel filtration, anion exchange chromatography, tag cleavage by recombinant TEV protease, desalting gel filtration, and another step of nickel affinity chromatography to remove the His-tag |