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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.19from cell-free extract
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.19recombinant enzyme
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.19recombinant enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by affinityy chromatography, desalting gel filtration, tag cleavage by human SenP2 protease, followed by another step of affinityy chromatography, and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.19recombinant His-tagged enzyme from Escherichia coli strain BL21 Gold (DE3) by nickel affinity chromatography, tag cleavage by a TEV protease mutant, and another step of nickel affinity chromatography, and dialysis of the flow through
Results 1 - 4 of 4