EC Number |
---|
1.13.12.13 | - |
1.13.12.13 | form inclusion bodies of recombinant Escherichia coli, solubilization with 6 M urea |
1.13.12.13 | recombinant His-tagged catalytic subunit mutant nanoKAZ from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis, wild-typeKAZ is purified from inclusion bodies using 6 M urea-nickel chelate affinity column chromatography |
1.13.12.13 | recombinant His-tagged catalytic wild-type and mutant enzymes and catalytic subunits from Escherichia coli by nickel affinity chromatgraphy |
1.13.12.13 | using Ni-NTA chromatography |
1.13.12.13 | using nickel-chelate affinity column |