EC Number   |
|---|
    1.13.11.49 | - |
| 1.13.11.49 | anion exchange column chromatography and S-200 HR gel filtration |
| 1.13.11.49 | cell pellet suspended in 0.05 M Tris-HCl buffer, pH 9.5 and 0.05 EDTA for 30 min at room temperature, centrifuged, supernatant ultracentrifuged, red supernatant containing periplasmic fraction separated from pellet, diluted with 10 mM Tris-HCl buffer, pH 7.2, purification with ÄKTAfplc, diluted supernatant loaded onto hydroxyapatite column with 10 mM Tris-HCl, pH 7.2, enzyme elutes at the start of a linear gradient of 10 mM Tris-Hcl, pH 7.2 to 450 mM potassium phosphate, pH 7.2, storage at -20°C |
| 1.13.11.49 | cell-free extracts are prepared from strain AW-1 cells grown in anaerobic medium with n-decane as sole carbon and energy source and chlorate as electron acceptor, centrifugation, storage of extracts under N2 gas at 4°C |
| 1.13.11.49 | centrifuged cell extract, cells grown in AW-1-sulfate medium with benzene and chlorate or benzene and oxygen |
| 1.13.11.49 | Co2+ chelating resin column chromatography |
| 1.13.11.49 | HiLoad 16/60 Superdex 200 prep-grade column, equilibrated with 20 mM Tris-HCl (pH 7.5) and 135 mM NaCl to determin molecular Stokes radius |
| 1.13.11.49 | His-trap affinity column chromatography and Superdex 200 gel filtration |
| 1.13.11.49 | HisTrap column chromatography |
| 1.13.11.49 | Ni Sepharose 6 column chromatography |
