EC Number |
---|
1.13.11.3 | - |
1.13.11.3 | a highly active enzyme without nuclease contaminations is purified by Ni-NTA affinity chromatography and size exclusion chromatography for prolonged single-molecule fluorescence imaging |
1.13.11.3 | ammonium sulfate, DEAE-cellulose |
1.13.11.3 | ammonium sulfate, DEAE-cellulose, Bio-gel agarose, DEAE-cellulose |
1.13.11.3 | ammonium sulfate, DEAE-cellulose, Sephadex G-200 |
1.13.11.3 | ammonium sulfate, DEAE-Sepharose, octyl-Sepharose, Phenyl-sepharose, recombinant enzyme |
1.13.11.3 | ammonium sulfate, DEAE-Sepharose, Phenyl-Sepharose |
1.13.11.3 | ammonium sulfate, Q-Sepharose, partial purification |
1.13.11.3 | ammonium sulfate, Sephacryl S 300, DEAE-Sephadex A50 |
1.13.11.3 | cells harvested by centrifugation, washed twice with 50 mM sodium phosphate buffer, pH 7.0 containing 10% glycerol, disruption with solicitor, centrifuged, supernatant stored at -20°C |