EC Number |
Reference |
---|
1.13.11.20 | - |
674292, 710849, 758785 |
1.13.11.20 | 1 ml HisTrap HP column, metal ion (nickel) affinity chromatography, gel filtration, MonoQ 4.6/100 PE ion exchange column. The thioredoxin/6x His cysteine dioxygenase fusion protein separates into two apparent isoforms that elute as two distinct peaks, one that elutes at 50 mM imidazole and one that elutes at 100 mM imidazole during metal ion affinity chromatograohy. The protein in the second peak has a specific activity, that is 50-60% less than that of the protein in the first peak. In contrast to peak 1 peak 2 elutes as two pronounced peaks (A and B) from the MonoQ column. The cysteine dioxygenase in peak A has no detectabel activity. In the the standard cysteine dioxygenase purification procedure, only the form from peak 1 is retained and further purified. |
676962 |
1.13.11.20 | all buffers are supplemented with 5 mM dithiothreitol to prevent oxidation of the selenomethionine |
674591 |
1.13.11.20 | amylose column chromatography and Superdex 200 gel filtration |
711255 |
1.13.11.20 | HisTrap HP column, immobilized metal affinity chromatography |
674292 |
1.13.11.20 | HisTrap HP column, immobilized metal affinity chromatography, during purification of SCO30335 two peaks of highly purified recombinant protein are found to elute: one at 10% (peak1) and the second at 20% (peak 2), kinetic data show that peak 1 has lower Km and higher Vmax values for cysteine than peak 2 |
674292 |
1.13.11.20 | HisTrap HP column, immobilized metal affinity chromatography, during purification two peaks of highly purified recombinant protein are found to elute: one at 10% (peak1) and the second at 20% (peak 2), kinetic data show that peak 1 has lower Km and higher Vmax values for cysteine than peak 2 |
674292 |
1.13.11.20 | HisTrap HP column, immobilized metal affinity chromatography, during purification two peaks of highly purified recombinant protein are found to elute: one at 10% (peak1) and the second at 20%(peak 2), kinetic datat show that peak 1 has lower Km and higher Vmax values for cysteine thant peak 2. |
674292 |
1.13.11.20 | immobilized metal chelate affinity chromatography |
698981 |
1.13.11.20 | immobilized nickel affinity chromatography |
674569 |