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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.1.2.7-
Display the word mapDisplay the reaction diagram Show all sequences 1.1.2.7Ca2+-containing and Ca2+-free enzymes from cell-free extracts by ion exchange and hydrophobic interaction chromatography
Display the word mapDisplay the reaction diagram Show all sequences 1.1.2.7native active alpha2beta2 MDH complex by ultracentrifugation, anion echange chromatgraphy, and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 1.1.2.7native enzyme 22fold from strain AM1 in a single cation exchange chromatographical step, followed by ultrafiltration and buffer exchange, over 97% purity
Display the word mapDisplay the reaction diagram Show all sequences 1.1.2.7native enzyme 9fold to homogeneity by anion exchange chromatography, ammonium sulfate fractionation, and hydrophobic interaction chromatography, followed by ultrafiltration and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 1.1.2.7native isozymes by anion exchange chromatography and gel filtration, type I 7.9fold, type II 14.7fold, the lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press
Display the word mapDisplay the reaction diagram Show all sequences 1.1.2.7recombinant selennomethionine-labeled, detagged MxaJ(residues 12-281) without signal peptide from Escherichia coli strain BL21(DE3) by anion-exchange chromatography and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 1.1.2.7soluble fraction concentrated with Centricon (Millipore, Billerica, Mass, USA), applied to a POROS 20 HQ column, followed by FPLC Superose 12 HR 10/30 column
Results 1 - 8 of 8