EC Number   |
Reference |
|---|
   1.1.1.357 | - |
654626, 761761 |
| 1.1.1.357 | in one step using metal chelate chromatography, purity of the protein is more than 98% |
725335 |
| 1.1.1.357 | Q-Sepharose column chromatography and Sephadex G-100 gel filtration |
724209 |
| 1.1.1.357 | recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, gel fitration, and ultrafiltration |
739797 |
| 1.1.1.357 | recombinant enzyme from cytosol of COS-1 cells by ultracentrifugation |
762009, 763517 |
| 1.1.1.357 | recombinant enzyme from Escherichia coli strain BL21(DE3) |
761761 |
| 1.1.1.357 | recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by glutathione affinity chromatography, cleavage of the tag by thrombin, and anion exchange chromatography |
743179 |
| 1.1.1.357 | recombinant His-tagged AKR1C3 from Escherichia coli strain BL21 |
740737 |
| 1.1.1.357 | recombinant His-tagged and untagged enzyme from Escherchia coli strain BL21(DE3) nickel affinity chromatography, and by deoxycholic acid affinity chromatography, respectively, the latter to 94-98% purity. Deoxycholic acid is linked to the resin Sepharose 4B with the aid of the spacers as cyanuric chloride and ethanediamine. Adsorption analysis by adapting the adsorption isotherm to a specific binding model, the Scatchard analysis model, overview. Comparison of purification methods between synthetic affinity medium and Ni2+-Sepharose column |
742972 |
| 1.1.1.357 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) |
760867 |
