EC Number   |
|---|
   1.1.1.23 | 55°C, 40% ammonium sulfate, Sephadex G-50, DEAE-cellulose, crystallization |
| 1.1.1.23 | 60°C, pH 5 treatment, ammonium sulfate precipitation, gel filtration, DEAE-cellulose, gel filtration |
| 1.1.1.23 | ammonium sulfate precipitation, DEAE-chromatography, L-histidinol-Sepharose 4B affinity chromatography |
| 1.1.1.23 | ammonium sulfate precipitation, heating, affinity chromatography, preparative gel |
| 1.1.1.23 | enzyme expressed in SF9 cells, cell homogenate, Mono Q FPLC |
| 1.1.1.23 | heating, pH 4.8, ammonium sulfate, DEAE-cellulose |
| 1.1.1.23 | recombinant enzyme by anion exchange chromatography and gel filtration |
| 1.1.1.23 | recombinant enzyme from Escherichia coli strain BL21(DE3) 40fold to homogeneity by anion exchange chromatography, ultrafiltration, gel filtration, and another step of anion exchange chromatography |
| 1.1.1.23 | recombinant enzyme fusion protein |
| 1.1.1.23 | recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by glutathione affinity chromatography, tag cleavage, and gel filtration, and recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration |
