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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 1.1.1.227Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.227native enzyme from strain TCU-HL1 by hydrophobic interaction and anion exchange chromatography, recombinant refolded enzyme from Escherichia coli
Show all pathways known for 1.1.1.227Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.227partial
Show all pathways known for 1.1.1.227Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.227partial, using Phenyl-Sepharose hydrophobic interaction, Q-Sepharose Fast Flow and Mono-Q anion-exchange columns
Show all pathways known for 1.1.1.227Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.227production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium
Show all pathways known for 1.1.1.227Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.227recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3)pLysSby nickel affinity chromatography
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