EC Number |
---|
1.1.1.159 | - |
1.1.1.159 | GST-Sefinose column chromatography |
1.1.1.159 | native enzyme 175fold by anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction and affinity chromatography |
1.1.1.159 | Ni-IDA affinity column chromatography |
1.1.1.159 | partial |
1.1.1.159 | partially purified by ion-exchange chromatography |
1.1.1.159 | recombinant enzyme from Escherichia coli by heat treatment at 55-60°C, polyethyleneimine treatment, ammonium sulfate fractionation, and gel filtration, followed by lyophilization |
1.1.1.159 | recombinant from Escherichia coli, high yield |
1.1.1.159 | recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, anion exchange chromatography, and gel filtration |
1.1.1.159 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by metal affinity chromatography |