EC Number |
---|
1.1.1.12 | - |
1.1.1.12 | native enzyme by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interation chromatography, and another different step of anion exchange chromatography, followed by gel filtration. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography |
1.1.1.12 | native enzyme by dialysis, anion exchange chromatography, ammoniuim sulfate fractionation, hydrophobic interaction chromatography, again dialysis and another different step off anion exchange chromatography, dialysis, and gel filtration, followed by repeating the last anion exchange chromatographic step. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography |
1.1.1.12 | partial |
1.1.1.12 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) |
1.1.1.12 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
1.1.1.12 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) |
1.1.1.12 | recombinant protein |
1.1.1.12 | recombinant protein, expression in Escherichia coli |
1.1.1.12 | recombinant proteins purified using Ni-agarose |