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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 5.4.3.6-
Display the word mapDisplay the reaction diagram Show all sequences 5.4.3.6Ni-NTA resin column chromatography, HiTrap Q column chromatography, and Superdex 200 gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 5.4.3.6purification is carried out by affinity chromatography with GST-Sepharose, the GST-tag is cleaved by using the PreScission protease
Display the word mapDisplay the reaction diagram Show all sequences 5.4.3.6recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
Display the word mapDisplay the reaction diagram Show all sequences 5.4.3.6recombinant His-tagged wild-type and mutant enzymes by nickel affinity chromatography
Display the word mapDisplay the reaction diagram Show all sequences 5.4.3.6recombinant tyrosine aminomutase
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