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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 4.3.2.2Display the word mapDisplay the reaction diagram Show all sequences 4.3.2.2normal and mutant enzyme S413P 34461
Show all pathways known for 4.3.2.2Display the word mapDisplay the reaction diagram Show all sequences 4.3.2.2Ni-affinity chromatography 664041
Show all pathways known for 4.3.2.2Display the word mapDisplay the reaction diagram Show all sequences 4.3.2.2- 1538, 1541, 34449, 34451, 34454, 34457, 665595, 749293
Show all pathways known for 4.3.2.2Display the word mapDisplay the reaction diagram Show all sequences 4.3.2.2using a Ni-NTA column 681441
Show all pathways known for 4.3.2.2Display the word mapDisplay the reaction diagram Show all sequences 4.3.2.2using a Ni-NTA column, the 6-histidine tag is removed by cleavage with thrombin, separation of thrombin and histidine-tag from the enzyme is achieved by using a Sephadex G-100 column 682657
Show all pathways known for 4.3.2.2Display the word mapDisplay the reaction diagram Show all sequences 4.3.2.2using nickel nitrilotriacetic acid-agarose 682733
Show all pathways known for 4.3.2.2Display the word mapDisplay the reaction diagram Show all sequences 4.3.2.2using nickel affinity chromatography 682738
Show all pathways known for 4.3.2.2Display the word mapDisplay the reaction diagram Show all sequences 4.3.2.2recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Bl21 by nickel affinity chromatography 690934
Show all pathways known for 4.3.2.2Display the word mapDisplay the reaction diagram Show all sequences 4.3.2.2Q-Sepharose column chromatography and gel filtration 706979
Show all pathways known for 4.3.2.2Display the word mapDisplay the reaction diagram Show all sequences 4.3.2.2Ni-NTA agarose column chromatography 707497
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