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EC Number
Commentary
Reference
bovine brain tubulin is purified by two cycles of polymerization and depolymerization
goat brain microtubule protein is isolated by two cycles of polymerization and depolymerization, MAP-free tubulin is purified from the microtubule protein by phosphocellulose chromatography
MAP-free tubulin is purified from the microtubule protein of goat brain by phosphocellulose chromatography
microtubules are prepared from freshly isolated lens epithelial fractions from mice
ovine brain tubulin is prepared from freshly-killed animals using phosphocellulose chromatography
purified to ca. 35%
recombinant His-tagged beta-tubulin mutant T238A from Saccharomyces cerevisiae by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by dialysis and nickel affinity chromatography, tag cleavage by thrombin, and dialysis
using a glutathione-Sepharose affinity column
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