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EC Number
Purification (Commentary)
3.5.4.13
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3.5.4.13
ammonium sulfate precipitation and DE-52 anion-exchange column chromatography
3.5.4.13
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, the protein quickly precipitates at concentration higher than 2 mg/ml
3.5.4.13
recombinant protein
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