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EC Number
Commentary
Reference
23 fold
63 fold
HisTrap column chromatography
immobilized metal affinity chromatography
native LacG1 to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange and hydroxyapatite chromatography, and ultrafiltration; recombinant His6-tagged enzyme LacG2 to homogeneity by nickel affinity and adsorption chromatography, and ultrafiltration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant soluble His-tagged wild-type and mutant PalHs from Escherichia coli by ultracentrifugation, anion exchange chromatography, and gel filtration
recombinant tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration
Results 1 - 10 of 11 > >>