Refine search

Search Purification (Commentary)

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 6 of 6
EC Number
Commentary
Reference
immobilized metal ion affinity chromatography
Ni2+-NTA resin column chromatography; recombinant enzyme
preparation of RoDH-4 contains a minor contaminating protein band. Therefore, the exact concentration of RoDH-4 is determined based on the calibration curve constructed from several concentrations of BSA loaded on to the same SDS/PAGE gel
RDH1 activity during purification is maintained only with phosphotidylcholine
Sephacryl S300 gel filtration
solubilized enzyme is extremely unstable and loses almost 80% of the initial activity even at partially purified stage, purification uses resistance of enzyme to proteolysis at 4°C in the presence of 1% Triton
Results 1 - 6 of 6