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Search Purification (Commentary)

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Results 1 - 6 of 6
EC Number
immobilized metal ion affinity chromatography
Ni2+-NTA resin column chromatography; recombinant enzyme
preparation of RoDH-4 contains a minor contaminating protein band. Therefore, the exact concentration of RoDH-4 is determined based on the calibration curve constructed from several concentrations of BSA loaded on to the same SDS/PAGE gel
RDH1 activity during purification is maintained only with phosphotidylcholine
Sephacryl S300 gel filtration
solubilized enzyme is extremely unstable and loses almost 80% of the initial activity even at partially purified stage, purification uses resistance of enzyme to proteolysis at 4°C in the presence of 1% Triton
Results 1 - 6 of 6