EC Number   |
|---|
   6.2.1.45 | recombinant mRFP-enzyme from Escherichia coli strain DH5alpha by combined anion exchange/affinity chromatography and gel filtration |
| 6.2.1.45 | recombinant N-terminally His6-tagged enzyme UBA5 from Escherichia coli by nickel affinity chromatography |
| 6.2.1.45 | recombinant protein |
| 6.2.1.45 | recombinant protein is purified by Ni-NTA His-Bind Superflow Sepharose and Strep-Tactin Sepahrose |
| 6.2.1.45 | recombinant protein, HIS-Select nickel affinity chromatography, further steps, ion exchange chromatography, followed by gel filtration |
| 6.2.1.45 | recombinant tagged full-length wild-type enzyme and truncated mutant versions from Escherichia coli by affinity chromatography |
