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EC Number	Application	Commentary	Reference
6.3.2.9	analysis	assay for monitoring enzyme activity based on the accumulation of adenosine 5'-diphosphate, a product of the reaction catalyzed by MurD ligase, by conversion to a fluorescent signal via a coupled enzyme system, with counterscreen assay to eliminate false positive results	704728
6.3.2.9	drug development	MurD is a target for small molecule chemotherapeutics	692904
6.3.2.9	drug development	the enzyme is a target in the development of potential antibiotics designed to target the peptidoglycan biosynthetic pathway	746203

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Release 2023.1 (January 2023)