EC Number   |
Application |
Reference |
|---|
   4.4.1.4 | analysis |
development of a qRT-PCR assay that enables the quantification and normalisation of alliinase and cysteine synthase transcripts |
747815 |
| 4.4.1.4 | analysis |
Flow-injection enzymatic analytical system for determination of alliin based on the immobilized alliinase and an ammonia gas electrode provids linearity in the 1 × 10-5 to 1 × 10-3 mol/l alliin concentration range and exhibited good repeatability and operational stability |
701590 |
| 4.4.1.4 | analysis |
microwave inactivation of the enzyme alliinase, to eliminate the interference from alliinase sulfo products, is used for a rapid detection method for determination of herbicides in onions, overview |
692422 |
| 4.4.1.4 | biotechnology |
lachrymatory factor synthase and alliinase function in tandem, with the alliinase furnishing the sulfenic acid substrate on which the lachrymatory factor synthase acts. The lachrymatory factor synthase modulates the formation of biologically active thiosulfinates that are downstream of the alliinase in a manner dependent upon the relative concentrations of the lachrymatory factor synthase and the alliinase. These observations suggest that manipulation of lachrymatory factor synthase-to-alliinase ratios in plants displaying this system may provide a means by which to rationally modify organosulfur small molecule profiles to obtain desired flavor and/or odor signatures, or increase the presence of desirable biologically active small molecules |
706312 |
| 4.4.1.4 | drug development |
a monoclonal antibody (MAb) against Aspergillus fumigatus is produced and chemically ligated to the enzyme alliinase. The purified antibody-alliinase conjugate binds to conidia and hyphae of Aspergillus fumigatus at nanomolar concentrations. In the presence of alliin, the conjugate produces cytotoxic allicin molecules, which kills the fungus |
701748 |
| 4.4.1.4 | drug development |
Pure allicin is prepared by reacting synthetic alliin with a stabilized process of the garlic enzyme alliinase. A novel drug vector, polybutylcyanoacrylate (PBCA) nanoparticles has been developed by emulsion polymerization method and wrapps the pure allicin into it. Minimal inhibitory fungal concentration (MIC) and minimal fungicidal concentration (MFC) of pure allicin and PBCA-allicin NPs against six fungus strains (Candida albicans, Cryputococcus neoformans, Trichophyton rubum, Microsporum gypseum, Microsporum canis and Epidermophyton floccosum) in vitro are used to evaluate their anti-fungal efficacy |
704719 |
| 4.4.1.4 | medicine |
a chemical conjugate between daidzein and the enzyme alliinase specifically binds to ovarian cancer cells and upon addition of the prodrug alliin, effectively produces cytotoxic allicin molecules which kill the cancer cells. Tumors show a five fold higher uptake as compared to other tissues. Treatment of tumor bearing mice with daidzein-alliinase and alliin effectively attenuates tumor progression during the first 12 days while a 5-fold increase in bioluminescence is detected in placebo-treated animals. Autopsy revealed only small individual foci of luminescence at the site of tumor cells inoculation. Histological examination of organs and tissues did not reveal any additional foci of carcinoma or signs of toxicity |
715742 |
| 4.4.1.4 | medicine |
Allium sativum is used as a food, spice, and traditional medicine for many centuries. It has also antibacterial, antioxidant, anticancer and cholesterol-lowering effects, many health benefits associated with garlic can be attributed to thiosulfinates, especially allicin (2-propenyl 2-propenethiosulfinate) generated by the enzyme |
729464 |
| 4.4.1.4 | medicine |
major allergen of garlic |
665289 |
| 4.4.1.4 | medicine |
no clinical trial should be conducted with a garlic powder supplement that is not standardized upon dissolution allicin release for an effect of garlic that may be related to allicin, as most are |
655760 |
