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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.2.3.107analysis PCR-based approach to identify BAC clones for two target genes, 3-carene synthase and a cytochrome P450 from a non-arrayed genomic BAC library. Shotgun genomic fragments isolated from the BAC clones were sequenced to a depth of 15.6- and 16.0fold coverage, respectively. Assembly and manual curation yielded sequence scaffolds of 172 kbp long for 3CAR. Inspection of the genomic sequences reveals the intron-exon structures, the putative promoterregions and putative cis-regulatory elements. Sequences related to transposable elements, high complexity repeats and simple repeats are prevalent and comprise approximately 40% of the sequenced genomic DNA 717443
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