EC Number |
Application |
Reference |
---|
4.2.1.30 | analysis |
determination of cobamide derivates in picomole quantities |
-, 33821 |
4.2.1.30 | analysis |
determination of vitamin B12 in the range 0.057 to 1.0 pmol using glycerol dehydrase. The sensitivity and accuracy of this method are lower than those of other methods. But because the procedure for the method is simple, it can be performed in any laboratory in a short time. The method can be useful as a complement to other methods |
666916 |
4.2.1.30 | biotechnology |
development of an economical and eco-friendly biological process for the production of 1,3-propanediol by an operon harboring the dhaB1, dhaB2, and yqhD genes, from renewable resources |
695764 |
4.2.1.30 | synthesis |
1,3-Propanediol is a valuable chemical intermediate,which is particularly suitable as a monomer for polycondensations to produce polyesters, polyethers and polyurethanes |
672728 |
4.2.1.30 | synthesis |
1,3-propanediol, starting material for producing plastics, from glycerol relies on the activity of glycerol or diol dehydratases |
33838 |
4.2.1.30 | synthesis |
7.4fold increased production of 3-hydroxypropionic acid from glycerol in the DELTAtpiA DELTAzwf DELTAyqhD Escherichia coli strain by the expression of glycerol dehydratase from Klebsiella pneumoniae and aldehyde dehydrogenase |
748579 |
4.2.1.30 | synthesis |
enzymatic production of 1,3-propanediol, which is a compound of great potential application in many synthesis reactions, in particular as a monomer for polycondensations to produce polyethers, polyurethanes, and polyesters. An optimal PCR system is developed for effective and reproducible PCR amplification of unknown long gene segments from the genome. Using this method, a new glycerol dehydratase is obtained, which eliminates the need for coenzyme B12. This reduces the cost of 1,3-propanediol production from renewable resources |
-, 747653 |
4.2.1.30 | synthesis |
Escherichia coli is engineered to produce 1,3-propanediol from glycerol, an inexpensive carbon source. This is done by introducing a synthetic 1,3-propanediol production pathway in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli. When 10 mM succinate is added to the medium, the titer of 1,3-propanediol and the glycerol consumption increase to 9.9 and 23.84 g/l, respectively. In addition, the ratio of NADH to NAD+ increases by 43%. Succinate addition is a promising route for the biotechnological production of NADH-dependent microbial metabolites |
747360 |
4.2.1.30 | synthesis |
glycerol dehydratase is a key and rate-limiting enzyme in the bioconversion process of glycerol to 3-hydroxypropionaldehyde, which is further reduced to 1,3-propanediol by 1,3-propanediol dehydrogenase. 3-Hydroxypropionaldehyde is an important chemical industry material, being a potent bacterial inhibitor and precursor in the production of numerous industrial chemicals, such as 1,3-propanediol |
-, 746790 |
4.2.1.30 | synthesis |
glycerol dehydratase is a key enzyme for the production of 1,3-propanediol in soluble cell extract. Klebsiella pneumoniae J2B shows a high potential for the production of 1,3-propanediol from glycerol. Optimization of the culture conditions and the elimination of lactate synthesis improves 1,3-propanediol production significantly |
-, 747304 |