4.1.1.46 | synthesis |
construction of an artificial pathway for muconic acid production in Escherichia coli by connecting 2,3-dihydroxybenzoic acid biosynthesis with its degradation pathway. Overexpression of entCBA and the key enzymes in the shikimate pathway leads to the production of 900 mg/l of 2,3-dihydroxybenzoic acid. Expression of 2,3-dihydroxybenzoic acid decarboxylase coupled with catechol 1,2-dioxygenase achieves the conversion of 2,3-dihydroxybenzoic acid into muconic acid. Assembly of the total pathway results in the de novo production of muconic acid up to 480 mg/l |
747588 |