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EC Number Application Commentary Reference
Display the reaction diagram Show all sequences 3.1.3.72agriculture partially purified enzyme shows properties that are suitable for animal feed applications 681220
Display the reaction diagram Show all sequences 3.1.3.72agriculture useful as food and animal feed supplement 682641
Display the reaction diagram Show all sequences 3.1.3.72synthesis transgenic expression in Pichia pastoris. Increasing isoform Alp2 copy number is detrimental to heterologous expression, clones with one copy of wild-type Alp2 produce the highest activity, clones with two, four and seven or more copies produce 70%, 25% and 10% respectively, of enzyme activity. Use of a sequence-optimized Alp2 gene increases the yield of the active enzyme by 25-50% in one/two copy clones. Reducing the temperature during heterologous expression leads to increases of 1.2-20fold suggesting that protein folding and post-translational processes may be the dominant factors limiting ALP2 expression 730874
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