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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.30synthesis a chemoenzymatic model for the preparative-scale synthesis of a diverse array of GDP-fucose derivatives is reported 706511
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.30synthesis engineering of Escherichia coli for efficient production of 2'-fucosyllactose by introduction of the fkp gene coding for fucokinase/GDP-L-fucose diphosphorylase from Bacteroides fragilis and the fucT2 gene encoding alpha-1,2-fucosyltransferase from Helicobacter pylori to produce 2'-fucosyllactose from fucose, lactose and glycerol. The yield and productivity are further improved by deletion of the fucI-fucK gene cluster coding for fucose isomerase and fuculose kinase. Fed-batch fermentation of the final strain results in 23.1 g/l of extracellular 2'-fucosyllactose and a productivity of 0.39 g/l/h -, 737901
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.30synthesis enzymatic synthesis of GDP-fucose 645223
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.30synthesis functional expression of enzyme gene in Saccharomyces cerevisiae. The yeast cells synthesize GDP-L-fucose or GDP-D-arabinose upon exogenous addition of L-fucose or D-arabinose, respectively -, 722350
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