EC Number |
Application |
Reference |
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2.5.1.91 | synthesis |
batch and fed-batch production of coenzyme Q10 |
701831 |
2.5.1.91 | synthesis |
for biotechnological production of coenzyme Q10 in recombinant Escherichia coli, three genetic manipulations are performed: heterologous expression of decaprenyl diphosphate synthase (Dps) from Agrobacterium tumefaciens, deletion of endogenous octaprenyl diphosphate synthase (IspB), and overexpression of 1-deoxy-D-xylulose synthase (Dxs). Expression of the dps gene and deletion of the ispB gene in Escherichia coli BL21(DE3)DELTAispB/pAP1 allows production of CoQ10 only. Coexpression of the dxs gene increases the specific content of CoQ10 |
704772 |
2.5.1.91 | synthesis |
heterologous overexpression in Escherichia coli results in a level of ubiquinone-10 in the transformants that is greater than that of intrinsic ubiquinone-8 |
707412 |
2.5.1.91 | synthesis |
recombinant Escherichia coli harboring the decaprenyl diphosphate synthase gene produces CoQ10 as well as CoQ8 and CoQ9. The recombinant Escherichia coli harboring only the decaprenyl diphosphate synthase gene produces 0.21 mg/l of CoQ10, whereas Escherichia coli coexpressing decaprenyl diphosphate synthase and 1-deoxy-D-xylulose 5-phosphate synthase produces 0.37 mg/l of CoQ10. The CoQ10 fraction is increased from 15.86% for only decaprenyl diphosphate synthase to 29.78% for coexpression of decaprenyl diphosphate synthase and 1-deoxy-D-xylulose 5-phosphate synthase |
688309 |
2.5.1.91 | synthesis |
ubiquinone-10 production using Agrobacterium tumefaciens dps gene in Escherichia coli by coexpression system. The expression coupled by dps and ubiCA is effective for increasing UQ-10 production by five times than that by expressing single dps gene in the shake flask culture |
-, 705650 |