EC Number   |
Application |
Reference |
|---|
   2.4.1.82 | agriculture |
development of an F2 population with 168 individuals by crossing lineages PI603452, PI283327, PI200508 and NA5909. A three base pair deletion in raffinose synthase 2 gene (GmRS2-Glyma.06G179200) on PI200508 accession explains 69.61%, 51.81% and 31.96% of stachyose, raffinose and sucrose variation, respectively, and soybean with average stachyose content of 0.18% can be produced. The mutation can be used to increase sucrose and reduce raffinose and stachyose content without major changes in oil and protein |
756526 |
| 2.4.1.82 | agriculture |
in seeds of the galactinol synthase GS1/GS2 and raffinose synthase RFS5 mutant the timing of desiccation tolerance acquisition is delayed as compared to wild type. Seeds from GS1/GS2 overexpressing plants with high levels of galactinol, raffinose, and stachyose, and RS5 overexpressing plants possess more raffinose and stachyose but less galactinol compared to wild type. These lines show greater germination percentage and shorter time to 50% germination after desiccation treatment at 11 and 15 days after flower. The role of raffinose family oligosaccharides is time limited and mainly affects the middle stage of seed development by enhancing seed viability and the ratio of GSH to GSSH in cells, but there is no significant difference in desiccation tolerance of mature seeds |
757498 |
| 2.4.1.82 | agriculture |
significant environmental effects exist on sucrose and the raffinose family of oligosaccharies for both commodity-type soybeans and lines with altered carbohydrate profiles. Altered carbohydrate soybeans can produce a high sucrose ( above 8% dry weight) and low raffinose family of oligosaccharies (less than 1% total raffinose and stachyose) phenotype across different locations when plants have the appropriate maturity. The carbohydrate profile is affected by the environment, and cooler temperatures during the later pod-filling stages strongly correlate with increased sucrose and decreased stachyose, the desired profile changes |
757027 |
| 2.4.1.82 | synthesis |
use of an in vitro multienzyme system using sucrose synthase (SUS), UDP-glucose 4-epimerase (GalE), galactinol synthase (GS), raffinose synthase (RS), and stachyose synthase (STS) and intermedia UDP and inositol, which can be recycled, to synthesize raffinose,. The reaction system produces 11.1mM raffinose using purified enzymes under optimal reaction conditions and substrate concentrations. Optimization further increases raffinose production to 86.6 mM and enables the synthesis of 61.1 mM stachyose. The UDP turnover number reaches 337. Inositol in the reaction system is recycled five times, and 255.8 mM raffinose (128.9 g/liter) is obtained |
755858 |
