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EC Number Application Commentary Reference
Show all pathways known for 2.4.1.257Display the reaction diagram Show all sequences 2.4.1.257synthesis engineering of a synthetic pathway in Escherichia coli for the production of eukaryotic trimannosyl chitobiose glycans and the transfer of these glycans to specific asparagine residues in target proteins. Glycan biosynthesis is enabled by four eukaryotic glycosyltransferases, including the yeast uridine diphosphate-N-acetylglucosamine transferases Alg13 and Alg14 and the mannosyltransferases Alg1 and Alg2. By including the bacterial oligosaccharyltransferase PglB from Campylobacter jejuni, glycans are successfully transferred to eukaryotic proteins 720519
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