EC Number   |
Application |
Reference |
|---|
   2.4.1.248 | synthesis |
immobilization of enzyme for production of cycloisomaltooligosaccharides. Immobilization on Chitopearl BCW-3505 results in 35% remaining activity, on Chitopearl BCW-3005 35%, Chitopearl SH-3505 28%, and Diaion HP-20 on 22% remaining activity. The maximum cycloisomaltooligosaccharides yield in batch reactions at 0.2, 2 and 10% dextran was 28, 24 and 12%, respectively. The concentration of linear oligosaccharides, the byproducts in the reaction mixture, isgreater with the immobilized CITase than the free enzyme. The immobilized CITase is less thermostable than the free enzyme by about 10°C |
697653 |
| 2.4.1.248 | synthesis |
immobilization of enzyme on anion-exchange porous hollow-fiber membrane with a degree of enzyme multilayer-binding of 0.3-9.8 and production of seven- to nine-glucose-membered cycloisomaltooligosaccharides from dextran at a maximum yield of 28% in weight at a space velocity of 10 per h during the permeation of 2.0% w/w dextran solution across the enzyme-immobilized porous hollow-fiber membrane. Yield increases with increasing degree of enzyme multilayering |
-, 696913 |
| 2.4.1.248 | synthesis |
optimal conditions for production of cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) from starch are substrate concentration 1%, pH 5.5, temperature 55°C, 24 h reaction time, enzyme concentration 1 unit/g-dry solid, isoamylase 2500 units/g-dry-solid |
-, 696806 |
| 2.4.1.248 | synthesis |
the enzyme can be applied to produce relatively large CIs at high temperature |
758960 |
| 2.4.1.248 | synthesis |
use of enzyme for synthesis of cycloisomaltooligosaccharides from dextran. Enzyme immobilized on chitopearl BCW-3505 is not influenced by NaCl up to 2 M. When 2 M NaCl is included in the substrate solution during continuous production of cycloisomaltooligosaccharides by a column system packed with the immobilized enzyme, no microbial contamination appears and cycloisomaltooligosaccharides are produced for 40 days. The added NaCl has no influence on the life time of the system and is effective in suppressing the growth of contaminating microbes. The added NaCl can be separated easily from cycloisomaltooligosaccharides by an open column system packed with activated carbon |
696066 |
