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Results 1 - 4 of 4
EC Number Application Commentary Reference
Show all pathways known for 2.3.1.8Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.8biofuel production proteome analysis as well as enzyme assays performed in cell-free extracts demonstrates that glycerol is degraded via glyceraldehyde-3-phosphate, which is further metabolized through the lower part of glycolysis leading to formation of mainly ethanol and hydrogen. Fermentation of glycerol to ethanol and hydrogen by this bacterium represents a remarkable option to add value to the biodiesel industries by utilization of surplus glycerol 748573
Show all pathways known for 2.3.1.8Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.8biotechnology genetic tools for use in Clostridium thermocellum that allow creation of unmarked mutations while using a replicating plasmid. The strategy employs counter-selections developed from the native Clostridium thermocellum hpt gene and the Thermoanaerobacterium saccharolyticum tdk gene and is used to delete the genes for both lactate dehydrogenase (Ldh) and phosphotransacetylase (Pta) -, 735482
Show all pathways known for 2.3.1.8Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.8synthesis a lactate dehydrogenase (Ldh) and phosphotransacetylase (Pta) deletion strain is evolved for 2,000 h, resulting in a stable strain with 40:1 ethanol selectivity and a 4.2-fold increase in ethanol yield over the wild-type strain. In a coculture of organic acid-deficient engineered strains of both Clostridium thermocellum and Thermoanaerobacterium saccharolyticum, fermentation of 92 g/liter Avicel results in 38 g/liter ethanol, with acetic and lactic acids below detection limits, in 146 h. engineering is based on a phosphoribosyl transferase (Hpt) deletion strain, which produces acetate, lactate, and ethanol in a ratio of 1.7:1.5:1.0, similar to the 2.1:1.9:1.0 ratio produced by the wild type. The Hpt/Ldh double mutant strain does not produce significant levels of lactate and has a 1.4:1.0 ratio of acetate to ethanol. Similarly, the Hpt/Pta double mutant strain does not produce acetate and has a 1.9:1.0 ratio of lactate to ethanol. The Hpt/Ldh/Pta triple mutant strain achieves ethanol selectivity of 40:1 relative to organic acids -, 735482
Show all pathways known for 2.3.1.8Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.8synthesis overexpression of phosphotransacetylase in the yeast Rhodosporidium toruloides for enhanced cell growth and lipid production. Compared with the parental strain, the engineered strain shows significant improvement in glucose consumption, cell growth and lipid accumulation when cultivated under nitrogen limited conditions in an Erlenmeyer flask as well as a stirred tank bioreactor. The phosphotransacetylase enzyme has little direct effects on the enzymes of fatty acid biosynthetic pathway, but might facilitate the fatty acid precursor acetyl-CoA supply 758341
Results 1 - 4 of 4