EC Number   |
Application |
Reference |
|---|
   2.3.1.75 | agriculture |
transgenic plants expressing the enzyme allow the production of long-chain liquid waxes at reasonable cost for use in commercial applications |
487487 |
| 2.3.1.75 | analysis |
development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction |
-, 718651 |
| 2.3.1.75 | synthesis |
expression of a fusion protein between Marinobacter hydrocarbonoclasticus wax synthase and Marinobacter adhaerens fatty acyl-CoA reductase in Nicotiana benthaminana. Compared to wild-type controls, transgenic plants show both in leaves and stems a significant increase in the total level of wax esters, being eight-fold at the whole plant level. The profiles of fatty acid methyl ester and fatty alcohol in wax esters are related, and C16 and C18 molecules constitute predominant forms. Strong transformants display certain developmental aberrations, such as stunted growth and chlorotic leaves and stems. These negative effects are associated with an accumulation of fatty alcohols |
737286 |
| 2.3.1.75 | synthesis |
synthesis of fatty acid ethyl esters in Saccharomyces cerevisiae by gene integration into chromosomal delta sequences using repetitive transformation, which results in 1-6 copies of the integration construct per genome. The corresponding fatty acid ethyl ester production increases up to 34 mg/L, an about sixfold increase compared to the equivalent plasmid-based producer. The cassette in the yeast genome is stably maintained in nonselective medium after deletion of RAD52. Additional overexprerssion of genes encoding endogenous acyl-CoA binding protein and a bacterial NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase (gapN) leads to another 40% increase in fatty acid ethyl ester production |
735806 |
| 2.3.1.75 | synthesis |
the enzyme can be used for oleyl oleate production in plant seed oil |
-, 756250 |
