EC Number   |
Application |
Reference |
|---|
    2.3.1.226 | medicine |
carboxymethylproline synthases is a biocatalysts for preparing functionalised N-heterocycles in a diastereoselective fashion. The products can be converted into the respective bicyclic beta-lactams of potential application in the semisynthesis of stable beta-lactam antibiotics |
722026 |
| 2.3.1.226 | synthesis |
biocatalytic production of bicyclic beta-lactams with three contiguous chiral centres using engineered crotonases. Structurally guided substitutions of wild type carboxymethylproline synthases enable tuning of their C-N and C-C bond forming capacity to produce 5-carboxymethylproline derivatives substituted at C-4 and C-6, from amino acid aldehyde and malonyl-CoA derivatives. Use of tandem enzyme incubations comprising an engineered carboxymethylproline synthase and an alkylmalonyl-CoA forming enzyme (i.e. malonyl-CoA synthetase or crotonyl-CoA carboxylase reductase) can improve stereocontrol and expand the product range. Some of the prepared 4,6-disubstituted-5-carboxymethylproline derivatives are converted to bicyclic beta-lactams by carbapenam synthetase catalysis |
756506 |
| 2.3.1.226 | synthesis |
carboxymethylproline synthases is a biocatalysts for preparing functionalised N-heterocycles in a diastereoselective fashion. The products can be converted into the respective bicyclic beta-lactams of potential application in the semisynthesis of stable beta-lactam antibiotics |
722026 |
| 2.3.1.226 | synthesis |
coupling of methylmalonyl-CoA epimerase, crotonyl-CoA carboxylase reductase and carboxymethylproline synthase mutants in a three-enzyme one-pot sequential synthesis of functionalised C-5 carboxyalkylprolines starting from crotonyl-CoA and carbon dioxide |
735951 |
