EC Number   |
Application |
Reference |
|---|
    1.14.13.236 | analysis |
use of selective inhibitor phenylacetylene to differentiate toluene-degrading isolates from an aquifer |
-, 740861 |
| 1.14.13.236 | synthesis |
by manipulation of vectors and gene inserts to eliminate adventitious catalytic turnover of NADH, up to 60fold increase in the volumetric yield of T4moH activity can be obtained from recombinant fermentations in Escherichia coli BL21(DE3) |
-, 741364 |
| 1.14.13.236 | synthesis |
conversion of benzene to phenol and naphthalene to 2-naphthol in a two-phase system using whole cells expressing wild-type toluene 4-monooxygenase and the alpha subunit variant TmoA I100A. With mutant I100A, the solubility of naphthalene is enhanced and the toxicity of the naphthols is prevented by the use of a water/dioctyl phthalate (80:20, vol%) system More than 99%2-naphthol is extracted to the dioctyl phthalate phase, dihydroxynaphthalene formation is prevented, 92% 2-naphthol is formed, and 12% naphthalene is converted. Using 50 vol% dioctyl phthalate, and wild-type T4MO, a 51% conversion of benzene is obtained and phenol is produced at a purity of 97% |
-, 739929 |
| 1.14.13.236 | synthesis |
development of a two-phase (dioctyl phthalate/aqueous medium) culture system to obtain maximal indirubin from indole. A 50% (v/v) dioctyl phthalate two-phase system using tryptophan medium containing 3 mM cysteine, 5 mM indole, and 1 mM isatin yields 102.4 mg/l of indirubin with no conversion of indole to indigo |
740988 |
| 1.14.13.236 | synthesis |
improved synthesis of hydroxytyrosol in whole-cell biotransformation. An overall concentration of 133 mg/l hydroxytyrosol, corresponding to a volumetric productivity of 54 mg/l/h and a yield of 48% is achieved by a batch mode using 2 mM substrate. The use of beads conjugated with phenylboronic acid residues for adsorbing the product from the biotransformation bulk leads to 2fold purification resulting in 84% purity with 70% recovery yield |
-, 739900 |
| 1.14.13.236 | synthesis |
synthesis of hydroxytyrosol, a phenol present in olives, via double hydroxylation of 2-phenylethanol employing toluene monooxygenases as biocatalysts |
-, 740473 |
| 1.14.13.236 | synthesis |
synthesis of hydroxytyrosol, a phenol present in olives, via double hydroxylation of 2-phenylethanol employing toluene monooxygenases as biocatalysts. Mutant TmoA S395C shows a 15fold increase in 2-phenylethanol hydroxylation rate |
-, 740473 |
| 1.14.13.236 | synthesis |
transformation of 1 mM fluorobenzene by whole cells gives a 52% yield of 4-fluorocatechol as a single product. The yield is improved 1.6fold by adding 10 mM ascorbic acid to the biotransformations |
-, 740219 |
