EC Number   |
Application |
Reference |
|---|
   1.14.11.55 | synthesis |
an Escherichia coli cell factory expressing the Pseudomonas stutzeri ectD gene from a synthetic promoter imports homoectoine via the ProU and ProP compatible solute transporters, hydroxylates it, and secretes the formed trans-5-hydroxyhomoectoine, independent from all currently known mechanosensitive channels, into the growth medium |
-, 764733 |
| 1.14.11.55 | synthesis |
effect of medium formulation (i.e., yeast extract (YE) medium and high yeast extract (HYE) medium) on hydroxyectoine production. Hydroxyectoine production is elevated when the high yeast extract medium is utilized. Hydroxyectoine production increases to 2.9 g/l when 50 mM of 2-oxoglutarate and 1 mM of iron are added to the high yeast extract medium |
-, 765119 |
| 1.14.11.55 | synthesis |
efficient conversion of ectoine to hydroxyectoine in Halomonas elongata by heterologous expression of the ectoine hydroxylase gene, thpD, from Streptomyces anulatus under cotrol of the Hansenula elongata ectA promoter |
739896 |
| 1.14.11.55 | synthesis |
expression of 5-hydroxyectoine biosynthesis enzymes EctA, EctB, EctC, and EctD in Hansenula polymorpha. 5-Hydroxyectoine synthesis in gram per liter scale (2.8 g/l culture supernatant, 365 mol/g dry cell weight) can be achieved, with almost 100% conversion of ectoine to 5-hydroxyectoine without necessity of high salinity |
-, 740784 |
| 1.14.11.55 | synthesis |
in an optimized medium containing 100 g/l NaCl in a 500-ml flask, the double mutant lacking EctD and ectoine hydrolaseDoeA synthesizes 3.13 g/l ectoine after 30 h cultivation. Mutants additionally lacking key Na+/H+ antiporter Mrp can synthesize around 7 g/l or 500 mg/(g DCW) in the medium containing lower concentration of NaCl. During a fed-batch fermentation process with 60 g/l NaCl stress, a maximum 10.5 g/l ectoine is accumulated by the Mrp-deficient strain,with a specific production of 765 mg/(g DCW) and a yield of 0.21 g/g monosodium glutamate |
-, 765346 |
