EC Number   |
Application |
Reference |
|---|
   1.1.1.67 | analysis |
sensitive and specific photometric determination of mannitol in human serum |
655244 |
| 1.1.1.67 | biotechnology |
recombinant Escherichia coli expressing the enzyme from Leuconostoc pseudomesenteroides expressing strong catalytic activity of an NADH-dependent reduction of D-fructose to D-mannitol in cell extracts of the recombinant Escherichia coli strain can be utilized as an efficient biocatalyst for D-mannitol formation |
654309 |
| 1.1.1.67 | industry |
best strain for expression of MDH in both laboratory and industrial applications is Escherichia coli BL21 (DE3) plysS |
700553 |
| 1.1.1.67 | industry |
redox balancing between the intracellular NADP(H) and NAD(H) based on NAD(P)(H)-dependent interconversion of mannitol and fructose by M2DH may be a useful strategy of metabolic engineering |
686764 |
| 1.1.1.67 | industry |
the enzyme synthesizes D-mannitol is commonly known as a sweetener in food manufacturing such as juices, soft drinks, cakes, and sweet cookies, moreover, other processing including alternative sugar for diabetic patients, cosmetics, personal hygiene products, and laxative. D-mannitol is 50% as sweet as sucrose |
-, 763052 |
| 1.1.1.67 | nutrition |
- |
287260 |
| 1.1.1.67 | nutrition |
cofactor regeneration system |
287241 |
| 1.1.1.67 | synthesis |
an effective strategy for producing high yields of mannitol is developed. The combined strategies of aeration induction and redox modulation significantly increases the glucose consumption rate, intracellular NADH level and the specific activity of mannitol dehydrogenase (MDH), resulting in an increase in mannitol production from 64.6 to 88.1 g/l with the yield increased from 0.69 to 0.94 g/g |
-, 763338 |
| 1.1.1.67 | synthesis |
mannitol is a natural hexitol with important applications in medicine and food industry. Development of a production method on an industrial scale, optimization and evaluation of production in a batch reactor (BR, or BRP operation) for a complex bi-enzymatic system with suspended enzymes and cofactor regeneration, method modeling, molecular calculations and simulations, detailed overview |
-, 763682 |
| 1.1.1.67 | synthesis |
the enzyme might be useful for enzymatic D-mannitol production in an industrial scale. The purified mannitol dehydrogenase have been reported to produce D-mannitol with no sorbitol formation at temperatures of 90-120°C. The pathway for D-mannitol production using MtDH isolated from Thermotoga maritima involves production from glucose via Thermotoga neapolitana xylose isomerase (gene xylA, UniProt ID P45687) followed by the conversion of the formed D-fructose using Thermotoga maritima MtDH of enzymatic to chemical synthesis process, overview |
-, 763052 |
