EC Number   |
Application |
Reference |
|---|
   1.1.1.214 | synthesis |
enzyme is useful for production of chiral alcohols |
-, 654311 |
| 1.1.1.214 | synthesis |
in a continuous feeding reaction, 200 mM ketopantolactone is reduced to (R)-pantolactone with 98% conversion and 99% enantiomeric excess within 2.0 h |
-, 761008 |
| 1.1.1.214 | synthesis |
use of polyketone reductase (CPR), glucose dehydrogenase (GDH) and coenzyme NADP+ in organic-inorganic hybrid nanoflowers (hNFs) for the asymmetric reduction of ketopantolactone to synthesize (R)-(-)-pantolactone. The sodium alginate-coated hNF reactor successfully catalyzes the asymmetric synthesis of (R)-pantolactone, with satisfactory stereoselectivity and reusability in repeated batches |
-, 762360 |
| 1.1.1.214 | synthesis |
whole-cell biotransformation process to produce D-pantolactone in a biphasic reaction system. Recombinant CPR and glucose dehydrogenase are co-expressed in Escherichia coli to simultaneously achieve the synthesis of D-PL and the regeneration of NADPH. Presence of 15% dichloromethane significantly inhibits the hydrolysis of ketopantolactone. In a fed-batch system, the D-pantolactone concentration reaches 0.77 mol per l in the reaction mixture at 7 h, and its enantiomeric excess is 99% |
-, 762361 |
