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1.1.1.122
analysis
determination of bound-fucose in biological materials by a coupled enzymatic method in a single buffer system
285800
1.1.1.122
biotechnology
the purified alpha1,2-fucosidase and L-fucose dehydrogenase have sufficiently high activities in phosphate-buffered saline (pH 7.0) at 37 °C, making it possible to develop a one-pot method for the quantitative determination of 2'-fucosyllactose in fermentation samples. The application of this method is more convenient for quantifying 2'-fucosyllactose in a variety of samples that may be obtained from different phases of the biotechnological production of this oligosaccharide. The method is useful for simple and rapid screening of active variants during the development of any industrially important microbial strain producing 2'-fucosyllactose
-
,
762598
1.1.1.122
diagnostics
the purified alpha1,2-fucosidase and L-fucose dehydrogenase have sufficiently high activities in phosphate-buffered saline (pH 7.0) at 37 °C, making it possible to develop a one-pot method for the quantitative determination of 2'-fucosyllactose in fermentation samples. The application of this method is more convenient for quantifying 2'-fucosyllactose in a variety of samples that may be obtained from different phases of the biotechnological production of this oligosaccharide. The method is useful for simple and rapid screening of active variants during the development of any industrially important microbial strain producing 2'-fucosyllactose
-
,
762598
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