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construction of the trans-chrysanthemic acid biosynthetic pathway in tomato fruits by expressing the chrysanthemyl diphosphate synthase gene, as well as an alcohol dehydrogenease (ADH) gene and aldehyde dehydrogenase (ALDH) gene from a wild tomato species, all under the control of the PG promoter. Trangenic tomato fruits show a concentration of trans-chrysanthemic acid that is about 1.7fold higher (by weight) than the levels of lycopene present in non-transgenic fruit, the level of lycopene in the transgenic plants is reduced by 68%. Ninety seven percent of the diverted DMAPP is converted to trans-chrysanthemic acid, but 62% of this acid is further glycosylated
transient coexpression of CDS with ADH2 which encodes an enzyme that oxidizes trans-chrysanthemol to trans-chrysanthemal, and ALDH1 which encodes an enzyme that oxidizes trans-chrysanthemal into trans-chrysanthemic acid, in Nicotiana benthamiana leaves results in the production of trans-chrysanthemic acid as well as several other side products. The majority (58%) of trans-chrysanthemic acid is glycosylated or otherwise modified
Results 1 - 2 of 2