EC Number   |
Organic Solvent |
Reference |
|---|
   3.4.22.32 | 2,2,2-trifluoroethanol |
the enhanced binding of 1-anilino-8-naphthalene sulfonic acid to the specific/pre-molten globule (SMG) state of stem bromelain upon addition of 30% 2,2,2-trifluoroethanol suggests the presence of a large number of solvent-accessible non-polar clusters in the treated SMG. The formation of an molten globe (MG)-like state characterized by disordered side chain interactions but with considerable secondary structure when the specific/pre-molten globule (SMG) state of stem bromelain is subjected to 30% 2,2,2-trifluoroethanol (TFE). The TFE-induced MG conformation at alkaline pH could represent the conformation that allows stem bromelain to traverse membranes |
717671 |
| 3.4.22.32 | Ethanol |
20% v/v, 37°C, 20 min, 33% loss of activity |
30273 |
| 3.4.22.32 | Ethanol |
enzyme stabilized in 60-70% ethanol is non-functional, but retains all the elements of secondary structure |
680551 |
| 3.4.22.32 | guanidine-HCl |
6 M guanidine-HCl denatured state of stem bromelain is enzymatically inactive |
680545 |
| 3.4.22.32 | guanidine-HCl |
activity-enhanced state in the presence of 2 M guanidine-HCl |
680551 |
| 3.4.22.32 | Methanol |
25% v/v, 25°C, 20 min, stable |
30273 |
| 3.4.22.32 | urea |
activity-enhanced state in the presence of 5 M and 8-9 M urea |
680551 |
| 3.4.22.32 | urea |
stem bromelain is fully resistant against urea around neutral pH (5.5 to 10.0) and unfolds only below pH 5.0 |
707537 |
