EC Number   |
Organic Solvent |
Reference |
|---|
   3.4.21.B57 | 2-mercaptoethanol |
5%, reduces the activity by 80% |
-, 727459 |
| 3.4.21.B57 | dithiothreitol |
5%, reduces the activity |
-, 727459 |
| 3.4.21.B57 | guanidine-HCl |
4 mM, 14% residual activity |
-, 727459 |
| 3.4.21.B57 | guanidine-HCl |
Tk-subtilisin retains its specific activity in 6 M guanidine HCl after 60 min incubation at room temperature |
726790 |
| 3.4.21.B57 | SDS |
the enzyme retains 20% proteolytic activity |
-, 727459 |
| 3.4.21.B57 | SDS |
Tk-subtilisin shows no significant reduction of specific activity after 60 min incubation at room temperature with 5% SDS |
726790 |
| 3.4.21.B57 | urea |
4 mM, 44% residual activity |
-, 727459 |
| 3.4.21.B57 | urea |
Tk-subtilisin retains its specific activity in 8 M urea after 60 min incubation at room temperature |
726790 |
