EC Number |
Natural Substrates |
---|
4.1.99.14 | (5''R)-alpha-5''(6''H)-bithymine + S-adenosyl-L-methionine |
SPL repairs specifically the 5R isomer. (5''R)-alpha-5''(6''H)-bithymine is the diastereomer produced upon UV irradiation of a TpT dinucleotide |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine |
the enzyme establishes a complex radical transfer cascade and creates a cysteine and a tyrosyl radical dyade to establish repair. This allows the enzyme to solve topological and energetic problems associated with the radical based repair reaction |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine |
- |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine |
in double-helical DNA |
4.1.99.14 | (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double helical DNA) + S-adenosyl-L-methionine + 2 H+ |
- |
4.1.99.14 | 5,6-dihydro-5-(thymidin-7-yl)thymidine (in double helical DNA) |
- |
4.1.99.14 | more |
the (5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine lesion does not absolutely need to be contained within a single or double-stranded DNA for recognition and repaired by the enzyme |
4.1.99.14 | more |
DNA conformation during in vivo repair and dinucleotide SP TpT flipping process, overview |