EC Number |
Natural Substrates |
---|
3.2.2.24 | ADP-D-ribosyl-(dinitrogen reductase) component of nitrogenase complex + H2O |
ADP-ribosylation of initrogenase reductase (NifH) occurs in response to addition of ammonium to the extracellular medium and is mediated by dinitrogenase reductase ADP-ribosyltransferase (DraT) and reversed by dinitrogenase reductase glycohydrolase (DraG). The PII protein GlnZ is implicated in regulation DraG. DraG binds to both the uridylylated and deuridylylated forms of GlnZ |
3.2.2.24 | ADP-D-ribosyl-(dinitrogen reductase) component of nitrogenase complex + H2O |
nitrogenase activity in Rhodospirillum rubrum is post-translationally regulated by DRAG (dinitrogenase reductase glycohydrolase) and DRAT (dinitrogenase reductase ADP-ribosylation transferase). When a sudden increase in fixed nitrogen concentration or energy depletion is sensed by the cells, DRAG is inactivated and DRAT activated |
3.2.2.24 | ADP-D-ribosyl-[dinitrogen reductase] |
- |
3.2.2.24 | ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex |
- |
3.2.2.24 | ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex |
i.e. component II or Fe-protein, one of the two identical subunits of dinitrogen reductase |
3.2.2.24 | ADP-ribosyl-[dinitrogen reductase] + H2O |
- |
3.2.2.24 | ADP-ribosyl-[dinitrogen reductase] + H2O |
DraG removes ADP-ribose upon exposure of the bacteria to light or depletion of the nitrogen source added, regenerating the arginine guanidino group and activating the dinitrogenase reductase dimer |
3.2.2.24 | ADP-ribosylated Fe-protein |
- |
3.2.2.24 | more |
DraG is capable of auto ADP-ribosylation when in excess of ADP-ribose, this reaction can function as an autoregulatory mechanism in vivo |