EC Number   |
Natural Substrates |
|---|
   3.1.21.10 | DNA + H2O |
- |
| 3.1.21.10 | DNA + H2O |
holliday structure |
| 3.1.21.10 | DNA + H2O |
the enzyme is required for meiotic crossing over but not for gene conversion |
| 3.1.21.10 | DNA + H2O |
the enzyme prevents mitochondrial DNA aggregation in Schizosaccharomyces pombe |
| 3.1.21.10 | DNA + H2O |
in contrast to GEN1, MUS81-EME1 cleaves intact Holliday junctions poorly (preferring nicked Holliday junctions, 3'-flaps, and replication fork structures as its DNA substrates). SLX1-SLX4 and MUS81-EME1 cooperatively cleave Holliday junctions by a nick and counter-nick mechanism |
| 3.1.21.10 | DNA + H2O |
the enzyme cleaves a variety of DNA structures including intact Holliday junction and nicked and gapped duplex DNAs generating double-strand breaks. MUS81-EME2 cleaves two strands among three strands present in an nicked duplex. MUS81-EME2 preferentially cleaves nicked duplexes lacking a 5'-phosphate at the nick |
| 3.1.21.10 | DNA + H2O |
the enzyme from gene G44P preferentially cleaves Holliday junctions, but also, with lower efficiency, replicated D-loops |
| 3.1.21.10 | DNA + H2O |
the enzyme requires inherent rotational flexibility in DNA junctions for optimal catalysis. Recognition of 3'-flap and nicked Holliday junction substrates involves induction of a sharp bend with a 100° angle between two duplex DNA arms |
| 3.1.21.10 | DNA + H2O |
the substrate spectrum of MUS81-EME1 comprises 3'-flaps (duplex DNA with a 3'-single-stranded flap), double-stranded three-way junctions that resemble replication forks, Holliday junction precursors, and fully ligated Holliday junctions. Slx1Slx4 cleaves splayed arm DNA substrates (a duplex with unpaired 3'- and 5'-overhangs on one side), 5'-flaps (duplex DNA with a 5'-single-stranded flap), replication forks, and Holliday junctionss |
| 3.1.21.10 | DNA + H2O |
the enzyme is required for mtDNA transmission and affects mtDNA content |
