EC Number   |
Natural Substrates |
|---|
   3.1.13.1 | more |
Dis3 is responsible for exosome core activity |
| 3.1.13.1 | more |
RNase activity of Dis3 is required for proper kinetochore formation and establishment of kinetochore-microtubule interactions. Dis3 is suggested to contribute to kinetochore formation through an involvement in heterochromatic silencing at both outer centromeric repeats and within the central core region |
| 3.1.13.1 | more |
the enzyme is involed in processing of polycistronic tRNA transcripts. Polynucleotide phosphorylase (PNPase) and RNase II are required for the removal of the 3 Rho-dependent terminator sequences |
| 3.1.13.1 | more |
mRNA is a direct substrate for RNase R |
| 3.1.13.1 | more |
RNase R is a 3'-5'-exoribonuclease that is very processive and can efficiently digest RNAs having extensive secondary structures, such as rRNA, RNAs containing repetitive extragenic palindromic (REP) sequences, or the transfer-messenger RNA required for trans-translation. |
| 3.1.13.1 | more |
RNase R is also involved in the processing of 16S and 5S rRNA |
| 3.1.13.1 | more |
The absence of RNase R leads to moderate increases in the mRNA levels of some RNases and RNA helicases, but other RNases and RNA helicases are not affected. |
| 3.1.13.1 | more |
at optimal (37°C) or elevated (42°C) growth temperatures, the loss of RNase R in the RNase R mutant has no major consequence on bacterial growth and has a moderate impact on normal gene regulation. At lower temperatures (25°C or 30°C), the loss of RNase R has a significant impact on bacterial growth and results in the accumulation of structured RNA degradation products. Concurrently, gene regulation is affected and specifically results in an increased expression of the competence regulon. Loss of the exoribonuclease activity of RNase R is sufficient to induce competence development, a genetically programmed process normally triggered as a response to environmental stimuli. The temperature-dependent expression of competence genes in the rnr mutant is independent of previously identified competence regulators. The rnr mutant is competent for genetic transformation. RNase R is dispensable for the intracellular multiplication of Legionella pneumophila in both human and protozoan hosts. A physiological role of RNase R is to eliminate structured RNA molecules that are stabilized by low temperature, which in turn may affect regulatory networks, compromising adaptation to cold and thus resulting in decreased viability |
| 3.1.13.1 | more |
RNase II is one of the major enzymes involved in mRNA processing. If the CSD is limiting the action of RNase II in vivo, it may play an important role working as a brake and thus preventing the massive degradation of RNA |
| 3.1.13.1 | more |
RNase II degrades RNA hydrolytically in the 3' to 5' direction in a processive and sequence independent manner. RNase II activity is impaired by double-stranded RNAs |
