EC Number |
Natural Substrates |
---|
2.7.7.B22 | more |
formation of a Hermes transposase-DNA complex. Bipartite DNA recognition at hAT transposon ends and Hermes-DNA interactions within the transpososome. No protein-DNA interactions involving bp 12-16 of the TIR. The avidity provided by multiple sites of interaction allows a transposase to locate its transposon ends amidst a sea of chromosomal DNA, mechanism, overview. The enzyme possesses a RNaseH-like catalytic domain interrupted by a large [.alpha]-helical insertion domain, and an N-terminal intertwined dimerization domain. Together, these domains catalyze the chemical steps of DNA nicking, hairpin formation, and DNA strand transfer that comprise hAT transposition |
2.7.7.B22 | more |
H2K A/J muscle cells properly express full-length human DYSF following SB-mediated gene transfer. A duplicate of Spc5-12 (2xSpc5-12) regulatory sequence proves to be the most efficient in driving transgene expression in H2K A/J myoblasts. Corrected H2K A/J myoblasts can efficiently be transplanted into Scid/BLA/J mouse muscle |
2.7.7.B22 | more |
the sleeping beauty transposon DNA contains the gene to be inserted into the target DNA flanked by inverted terminal repeats (IR-DRleft and IR-DRright). Each inverted terminal repeat contains two, inner and outer, direct repeats (DRs). The DRs represent binding sites for the transposase enzyme. Sleeping beauty, SB, appears to be the most random in preferences for integration sites, requiring only a TA dinucleotide basepair. Binding mechanism, detailed overview |